Insertion junction: CLIP2.039455_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039455
Chromosome:chromosome 11
Location:2863000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095045 (1 of 1) PF00628//PF13871//PF13872 - PHD-finger (PHD) // C-terminal domain on Strawberry notch homologue (Helicase_C_4) // P-loop containing NTP hydrolase pore-1 (AAA_34) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGAGCGCCTCTGAGCTGCTTTGCCCCCACCAACATGCACAGCCCCGGC
Internal bar code:TACAGGTCTACTGAGTTGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:584
LEAP-Seq percent confirming:37.5
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CACAATGGCCTCGTCGTAGT
Suggested primer 2:ACAGTCGGCGTTTGTCATCT