| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039458 |
| Chromosome: | chromosome 14 |
| Location: | 348511 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610050 | (1 of 1) K15180 - negative elongation factor B (COBRA1, NELFB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGCGGACGAAGCCCACGGTATTGGCGAGCGGTCTGCAGCCCGCCCG |
| Internal bar code: | GTTACGCTGTAAAGATGAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCAGCGAGGAGGATAGC |
| Suggested primer 2: | TGTTTTAACGCGCACAAGGG |