Insertion junction: CLIP2.039461_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039461
Chromosome:chromosome 12
Location:5419431
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g529450 DRP5A,DRP5 (1 of 1) PTHR11566//PTHR11566:SF44 - DYNAMIN // DYNAMIN-LIKE PROTEIN C; Dynamin-type GTPase involved in plastid division intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCATGCACTCAATGTTCCGCACACGGTCCCCCAGGTCTGAATATCGG
Internal bar code:ACAACTGAATCTGGTTTGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1575
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAGCGGAACTGCGTATGT
Suggested primer 2:GGGGTCTCTCGTGCGATAAG