Insertion junction: CLIP2.039470_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039470
Chromosome:chromosome 16
Location:6591967
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g673950 (1 of 1) 2.6.1.80 - Nicotianamine aminotransferase / Nicotianamine transaminase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCGAGCCCAACCGCTTTGTTCACCAGTTGCCAGCTACGTTCCTCCCT
Internal bar code:GGGAAAAGTGTTAGTATGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1909
LEAP-Seq percent confirming:73.5849
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGGTTCGGCATCATTCG
Suggested primer 2:CTGGACTCGTGCTTGGTCTT