| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.039472 |
| Chromosome: | chromosome 16 |
| Location: | 7022400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673841 | (1 of 1) PTHR13481:SF0 - UPF0454 PROTEIN C12ORF49 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACAACGCCGTCAGCATAGCAAAGCAGGCGCTGAGGTGCGCCTGGGGT |
| Internal bar code: | AGGTTTTTTCATGATTCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3165 |
| LEAP-Seq percent confirming: | 94.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCACTTTCCCGGTAGT |
| Suggested primer 2: | CCGAGCGCGATTCAAGAAAG |