Insertion junction: CLIP2.039474_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039474
Chromosome:chromosome 6
Location:6129375
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTAGGGCCGGACTGTCCCGCACCCCACATCGCACCCGTGACTGTGTA
Internal bar code:GACGATGAGATTGAATAGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2610
LEAP-Seq percent confirming:38.0952
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GACAACTGCTGCTGGTGTTG
Suggested primer 2:GAGCAGGCTCTGGAAATGGT