| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039490 |
| Chromosome: | chromosome 3 |
| Location: | 6693318 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195600 | (1 of 1) 3.2.1.28 - Alpha,alpha-trehalase / Trehalase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACGGCAATACCGTAGATTCCTGAACGCAGCCGGAAACAGGGTGTGCC |
| Internal bar code: | CCGGTAGTCATGTTGGGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4153 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTAAGCACGTAAGCATG |
| Suggested primer 2: | GTCAGCAACAACCACAGCAG |