Insertion junction: CLIP2.039517_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039517
Chromosome:chromosome 4
Location:213039
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217150 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAATAATCGCCGCTGACGTCCACTGGCGTATAACAGAGCCCGCAAGCAG
Internal bar code:TGTGTATGTCTCCGCTGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:194
LEAP-Seq percent confirming:4.7619
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTCGGTTTTGCAGGCTG
Suggested primer 2:GTGGAGAGATCAGGAGGGGT