| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039546 |
| Chromosome: | chromosome 5 |
| Location: | 924215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246650 | (1 of 1) IPR023214//IPR027377 - HAD-like domain // Zinc-binding domain | 3'UTR | |
| Cre05.g800526 | 5'UTR | ||
| lncRNA_TCONS_00046746 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGACAAAGGGCGACAGCTGACGCACACCTTGTGGTCGTTCAGGATAC |
| Internal bar code: | CTAATTGTGTGTGTATTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2878 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 230 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTCTCGAAATCCCTGGC |
| Suggested primer 2: | TCGCTTCGTAGCCACTGTTT |