| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039553 |
| Chromosome: | chromosome 5 |
| Location: | 1014370 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247350 | (1 of 30) IPR000253 - Forkhead-associated (FHA) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGGCGGACGCGGTCCAAGCTTTCAGAGGCGAGGCGTAGCACCCGGCG |
| Internal bar code: | CTTCGCCAATTCGCTAGAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1338 |
| LEAP-Seq percent confirming: | 65.1163 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTTGTTTCCTACGTGCC |
| Suggested primer 2: | AGTTGCTGCTGTTGTTGCTG |