Insertion junction: CLIP2.039565_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.039565
Chromosome:chromosome 10
Location:3797108
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g445650 SMC3 (1 of 1) K06669 - structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6) (SMC3, CSPG6); Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTAAATGGAATCCGCTAAGCCCCAGATCATGACAACCTGTGGGTGCA
Internal bar code:ACTTCGGGTGTCTAACGGGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1658
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGAACAGGGTGTACTGCA
Suggested primer 2:GATCATGCTGACCTCTCCGG