| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039566 |
| Chromosome: | chromosome 11 |
| Location: | 906843 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467654 | (1 of 1) K13117 - ATP-dependent RNA helicase DDX35 [EC:3.6.4.13] (DHX35) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGCTGGTGGCAACCACCCCCAACCCCCAACGTCCGTCACAAATCCTC |
| Internal bar code: | GGCGTGAGCGAAGTACTGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 28.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTTGGAGAGATGGTGG |
| Suggested primer 2: | CACGAATGTAACCCCACCCA |