Insertion junction: CLIP2.039569_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039569
Chromosome:chromosome 13
Location:2796040
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g582650 MAPK1,LF4 (1 of 1) K08830 - renal tumor antigen [EC:2.7.11.22] (RAGE, MOK); Mitogen-activated protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTACTTGTTCACGCCCACGCCGCCCACGCCCACCGCGCCGCCGCCGC
Internal bar code:TTTAACTACGTTGTAGAGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CACGATCTATAACGCCGCCT
Suggested primer 2:CCGGAATGGTTTCTCTGCCT