| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039603 |
| Chromosome: | chromosome 3 |
| Location: | 6164907 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800356 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGATCGAGGAAACACGCAAGTGACGGCGTGCTATCCGGACCCGGTCTT |
| Internal bar code: | TAGCGACCGTTAGTAATGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGGCTCAAGTCAGAGG |
| Suggested primer 2: | CTTGTGCAGGGTCAGTAGCA |