Insertion junction: CLIP2.039626_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039626
Chromosome:chromosome 2
Location:991515
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079700 PYR2 (1 of 1) 2.1.3.2 - Aspartate carbamoyltransferase / Carbamylaspartotranskinase; Aspartate carbamoyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTACGCGGCTCAACAAGCTCCTCAGCGCCAGTCGACTTGTTAGGTCCAG
Internal bar code:TTATTTTGGCGCCAAGATATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4945
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTGATCTGGCGGTAACA
Suggested primer 2:ATGGGACGCGTCATGTTCTT