| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039655 |
| Chromosome: | chromosome 3 |
| Location: | 6158691 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190900 | (1 of 5) PTHR10351:SF42 - LD40663P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTCCGGGCCGGCGCCGCTGGACGACACGTACGGCCCCTACGTGCGC |
| Internal bar code: | ATTTAATGGCCGTCTTCCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1649 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGCTGGATGTTGTACT |
| Suggested primer 2: | AGCTGTTGATGCGTACGGAA |