Insertion junction: CLIP2.039658_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.039658
Chromosome:chromosome 3
Location:5676750
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g186600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATTTGCCTAGGTGCGCGGGCAGCTGAGGGCGGGCAGCTGAGGGAGGGA
Internal bar code:TATCGACGTGAGGACAACGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACACATGTCCCCAAACA
Suggested primer 2:GGAGGAGACATACAGGTGCG