Insertion junction: CLIP2.039677_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.039677
Chromosome:chromosome 6
Location:5599815
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287450 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGTGACGTTGTTCGAAGTGCAACAACACAGTAAAAGCACTTCATTGC
Internal bar code:ACCAGTTACTTCGGTGTATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTTCACACAGTCCCGCAC
Suggested primer 2:GCACTTGTGACATTGCCGTT