| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039690 |
| Chromosome: | chromosome 8 |
| Location: | 1125295 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362750 | (1 of 1) K01173 - endonuclease G, mitochondrial (ENDOG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTCCGAGCGCTCGCTGTGCGGCATGGCGTGGCGTGTCGGGGGCAGCA |
| Internal bar code: | CCAATGGTGTGGCTGGGGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2528 |
| LEAP-Seq percent confirming: | 65.5914 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGGGGCTGTGTTGATG |
| Suggested primer 2: | ACGGTATGGTGAGCGCTATG |