Insertion junction: CLIP2.039700_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039700
Chromosome:chromosome 1
Location:3906268
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025700 (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGTGAACTCCTCCCACGTCTGCGGCACCGTCAGGTTGTCGCGCTCGA
Internal bar code:CCACGTTGGGGATTCGCAAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:3170
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:113

Suggested primers for confirmation by PCR

Suggested primer 1:GGACCGGGCAATTTAGGACA
Suggested primer 2:CGAAGATGGTGCGCATCATG