| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039732 |
| Chromosome: | chromosome 11 |
| Location: | 3568403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477950 | (1 of 1) IPR000104//IPR005502 - Antifreeze protein, type I // ADP-ribosylation/Crystallin J1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGCCCTGGCGGCGGCCACTGGTCAGGCGCGTGACGCTGCGGCGGGC |
| Internal bar code: | CTGTGGCCTCCGGCGCGACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2258 |
| LEAP-Seq percent confirming: | 89.0909 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTGTACCGGTGTGGCAC |
| Suggested primer 2: | TCACCTCGTTTGCCACCAAG |