Insertion junction: CLIP2.039736_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039736
Chromosome:chromosome 3
Location:5505557
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g184800 (1 of 1) 3.6.1.42//3.6.1.6 - Guanosine-diphosphatase / GDPase // Nucleoside diphosphate phosphatase / Nucleoside-diphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGGCCGGTCGGCGTACGCGGACAGGCCAGGCTCTATGGCGCTAGAGGG
Internal bar code:GTGTCTTTAGACGACGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3430
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGACACTGCTGTCCCTC
Suggested primer 2:TGACCACACACACACACACA