Insertion junction: CLIP2.039772_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.039772
Chromosome:chromosome 16
Location:7225448
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677093 (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTCAAACCTACCTCTCGTGCGCGCACAGGCGCTCTTCGACTCCATGG
Internal bar code:TGAGTCGTCGGTTTAATCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:5791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCACAACGATACAGGACA
Suggested primer 2:GGACACGTACGTTGAGGGTT