Insertion junction: CLIP2.039780_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.039780
Chromosome:chromosome 17
Location:1820340
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709300 (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAACGACTTTGACGAGTCCTTTGTCGCCAACTTCCTGTACGACATGT
Internal bar code:GAGGTGTCTTGCCGATCTTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1263
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTGCCCGAGTAGAACTT
Suggested primer 2:GGCCAACCAGTCCATCATCA