| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039803 |
| Chromosome: | chromosome 10 |
| Location: | 4800952 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453050 | CYCM1 | (1 of 2) PTHR10026:SF51 - CYCLIN-K; Chlamydomonadale-specific cyclin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGAACGACAGCGCGATGACGAACCTCTCGCTGCAGTACGCTAGCTCG |
| Internal bar code: | CAAGAATGGGATTGGTGCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2921 |
| LEAP-Seq percent confirming: | 60.7143 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCATAGAGCTCGTTGA |
| Suggested primer 2: | GGGTGTACGAGACGGCTATG |