Insertion junction: CLIP2.039804_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.039804
Chromosome:chromosome 17
Location:1146507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802033 CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCTCTCGCCCATCCACCAGGCTGCGGTAGGTGTTGTGCAGTTCGTCG
Internal bar code:CTATCGTGTAAGTTAGAGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:958
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCTCGTTCTCCATCTC
Suggested primer 2:CATCCATCTCGTCCAGCTCC