| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039828 |
| Chromosome: | chromosome 2 |
| Location: | 5966187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118125 | 5'UTR | ||
| Cre02.g118151 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGATGTACTTGCGGACGCATGGATTGCTTTCCCCACTTGTATTCCTC |
| Internal bar code: | ACTTGGTCAGATTTTTCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2277 |
| LEAP-Seq percent confirming: | 73.0337 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCTGGCGACATTTCCT |
| Suggested primer 2: | CCACGCTATGGGACGTACTC |