| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039839 |
| Chromosome: | chromosome 10 |
| Location: | 4120060 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801167 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAACCTTGCCTTGCTGCTCTGCTGGCGCGTGTCCCTCGCTGCAGCCC |
| Internal bar code: | TGCCGTGCAAAAACCTTAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2785 |
| LEAP-Seq percent confirming: | 82.5397 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCGTGTTGCTGCCATAG |
| Suggested primer 2: | ACCAGCCTCCTGATGTTTCG |