Insertion junction: CLIP2.039850_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039850
Chromosome:chromosome 1
Location:3957556
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025950 PPR8 (1 of 1) IPR000209//IPR002885 - Peptidase S8/S53 domain // Pentatricopeptide repeat; PentatricoPeptide Repeat protein 8 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTGCGCACCAGCTCGCTCAACACCAAGAAGAGCGCCCCCAACCGCG
Internal bar code:TGACTCATCCGATGTTATGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:3.44828
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACGCCTTTATGACTGTG
Suggested primer 2:GCCCTTGAAGACTGACGACA