| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039852 |
| Chromosome: | chromosome 6 |
| Location: | 1764526 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263100 | (1 of 2) PF10263 - SprT-like family (SprT-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACCAGTGGTGGGTGCGGTAGTTATTGACCTCGCCGTGCATGCTGTGA |
| Internal bar code: | AAAGGCGGCGGTGCGTCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2866 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGCTACTCAAGGTAGGA |
| Suggested primer 2: | ATGCCTTTTTCTTGCCTGCG |