Insertion junction: CLIP2.039858_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.039858
Chromosome:chromosome 5
Location:1077026
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGGGCGGCCCGAACGCGTTTCCCAACTCCCCAACTCTCCTCTCCTCT
Internal bar code:AGAGTAGAATACAGCATTATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2762
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:146

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAGCATCCCTAATCGCT
Suggested primer 2:AAAGCACTCTCGCCTCTTCC