Insertion junction: CLIP2.039860_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039860
Chromosome:chromosome 16
Location:833375
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g801884 (1 of 1) K19001 - ATP-dependent DNA helicase (HELLS, DDM1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCCTTCCTCTGCACCTTCCCCCCCTCACCTCCCCAGCGCCTGCTGCC
Internal bar code:CATGACTGATATTAGCGCCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1385
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAAACAACAACACGCCGC
Suggested primer 2:GGAGATCAGCGAGGACGAAG