| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039860 |
| Chromosome: | chromosome 16 |
| Location: | 833377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801884 | (1 of 1) K19001 - ATP-dependent DNA helicase (HELLS, DDM1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCATGCCAGCTCAAATTGAACCAAGCGGGGAGGAAGGGGAGGGGGT |
| Internal bar code: | ATGAGAGGCAGGAGCCTTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 27.7778 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATCAGCGAGGACGAAG |
| Suggested primer 2: | CAAAAACAACAACACGCCGC |