| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.039864 |
| Chromosome: | chromosome 12 |
| Location: | 9369647 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541700 | OPR54 | OctotricoPeptide Repeat protein 54 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTCCGCCACCAGCGCCACGTCCAGCCCCCGCACCAGCGCCGCCACT |
| Internal bar code: | AGCCGTTGATTAGCCTATTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGCATCCTAGCCTCACT |
| Suggested primer 2: | CGCTGTTGTGCATCCATACG |