| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039888 |
| Chromosome: | chromosome 12 |
| Location: | 8785670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547100 | CGL2,SMM43 | (1 of 2) PTHR10108//PTHR10108:SF788 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; putative methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGCTGCCAGCAGCCTGCAGCAAACAAGCGCAGCAATGCAGCGGGAA |
| Internal bar code: | AAGATGCTCCTTATTGTGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1460 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGGGGCAAACTAGCATT |
| Suggested primer 2: | CTCGGATAACGGGTGTACGG |