| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039903 |
| Chromosome: | chromosome 8 |
| Location: | 2227055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAAAGACCCGCAGCTGGGGTCGTGGACAGGCTTATCTCCTTACACG |
| Internal bar code: | ACCAGATGTGACGCCGGCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGGGCGAGAGATCTT |
| Suggested primer 2: | GAGTAGGAGGAAGCGATGGC |