| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039916 |
| Chromosome: | chromosome 3 |
| Location: | 3002844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163300 | CDT1 | (1 of 1) PTHR28637:SF1 - DNA REPLICATION FACTOR CDT1; DNA replication initiation factor, CDT1-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATTTGGGGGATTGGAGCTTCTCTACAAGGCTTGTGACGCCGATGCGG |
| Internal bar code: | TGCGCGTTCAGTATTTCCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGCTGTTGTAGGTGACC |
| Suggested primer 2: | TTTATGGCAGCTGGTCTGGG |