Insertion junction: CLIP2.039925_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039925
Chromosome:chromosome 12
Location:9811390
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g547302 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGCGTGTTCGCGCTCTCCAGCGTCAAGCCTCCGGGGGAGCCGGGGC
Internal bar code:TTCTGCGGGTTCGGTGTGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:302
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTACTTACGGTGGCTCC
Suggested primer 2:GCTGAAAGGGATCTGCTGGT