| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039949 |
| Chromosome: | chromosome 2 |
| Location: | 3979643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102650 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACCCAGCATTCCATCTGTGCCACGCCAATGCCGCCTGGCGGCGGTG |
| Internal bar code: | CAAAATGATCTGATCTAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3700 |
| LEAP-Seq percent confirming: | 74.0385 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTACATTGGCATGCTGG |
| Suggested primer 2: | CACCAGCAGCACCACTTCTA |