Insertion junction: CLIP2.039960_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039960
Chromosome:chromosome 15
Location:1185016
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639100 FAP195 Flagellar Associated Protein 195 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACCGGTGCACGCCTGGGCAGCTGCTGCGCACCTGCGCCGGGAGCTTG
Internal bar code:ATAGGCGATCTAGGCGTCAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1499
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATACCCCAGTTTTCGCT
Suggested primer 2:AGGGGTAGGTAGGAGGCAAG