Insertion junction: CLIP2.039967_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039967
Chromosome:chromosome 1
Location:7579576
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g053200 (1 of 1) PTHR12415:SF0 - TYROSYL-DNA PHOSPHODIESTERASE 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCACCTCTGTTGTGCAGAGTCCGGCGGTGGGGGCATCTAGGCGCCCGC
Internal bar code:TAAGTCTTGTACTTGACGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCATCAACCGCACCTGTA
Suggested primer 2:TCTACCACTGCTGCTGTGTG