Insertion junction: CLIP2.039970_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039970
Chromosome:chromosome 15
Location:1288738
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGCAGCCAAACACACCGTGTGTGCGATTGATCGACGATGCTTTGTG
Internal bar code:TAAGGTAGTACGGATCTGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGCGATCAACAACAGTAC
Suggested primer 2:TGGGTTCGGTTTAGTTCGGG