Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.039972 |
Chromosome: | chromosome 12 |
Location: | 7407937 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g557350 | FAO9 | (1 of 1) PF00355//PF01266 - Rieske [2Fe-2S] domain (Rieske) // FAD dependent oxidoreductase (DAO); FAD-dependent oxidoreductase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGCAACCCCCCCTCCTTTCCTCCACGCGCCCCTCACCGCGGCGTGT |
Internal bar code: | AATCCGGGGTGCTAGAGGGCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1124 |
LEAP-Seq percent confirming: | 41.9355 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTACGGGGTTTGGTCAAG |
Suggested primer 2: | GAGTGTGTGTGGAGAAGGGG |