| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039974 |
| Chromosome: | chromosome 3 |
| Location: | 8615443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACGTCGCCGCTGACACTTCTTTGTGCGCCCCTCTCTTGCTCTCCCTT |
| Internal bar code: | GTTCAATACCTCGAGGATTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1303 |
| LEAP-Seq percent confirming: | 9.52381 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTTTCACTGACGAGGGT |
| Suggested primer 2: | CGCGGCCATAAGAAACTTCG |