| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.039987 |
| Chromosome: | chromosome 12 |
| Location: | 3193529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500150 | ALDH2E1,ALD5,ALDH2 | (1 of 1) K00128 - aldehyde dehydrogenase (NAD+) (E1.2.1.3); Aldehyde dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGCCTACACCTTCCACGAGCCGCTGGGCGTGGTGGGGCAGGTGCGT |
| Internal bar code: | CTTTGCTCCTCAGAAAATATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAACATCCCCTCCGCAC |
| Suggested primer 2: | GTACGGTACTTAGTCGGCGG |