| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040000 |
| Chromosome: | chromosome 4 |
| Location: | 260306 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217550 | EIF3C | (1 of 1) K03252 - translation initiation factor 3 subunit C (EIF3C); Eukaryotic translation initiation factor 3, subunit C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACCCACCTCCTCCCCTCCCCCACTACCACAGGAGAACCCCAACCTC |
| Internal bar code: | CGTTAACCGGGACATCAAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCTTGAACAGCTCGTC |
| Suggested primer 2: | TCTCTGCTCCCCCATGATCA |