Insertion junction: CLIP2.040015_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040015
Chromosome:chromosome 16
Location:1671866
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654600 PHC56 (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTCGCACGTGAGTCTCAAGGGATGCCACGAGCACAACTGACACGCCGC
Internal bar code:ATCGAGACTGAGGGTATGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGACATCATCGTGGGCG
Suggested primer 2:TGTGTCAAGGCAAGCAAGGA