Insertion junction: CLIP2.040051_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040051
Chromosome:chromosome 3
Location:4933422
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179000 RAA16 component psaA trans-splicing sub complex II outside_mRNA
Cre03.g800341 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGACCGCCTGGGAGTACCACGTCCGCCGCCAACTCAACGACGCAGCC
Internal bar code:TGGAATAAACGTAACGCAAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2375
LEAP-Seq percent confirming:90.3226
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTTTGGTTCAAAGGCAT
Suggested primer 2:CGGGTAGCTCGCTATTCGTT