Insertion junction: CLIP2.040085_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040085
Chromosome:chromosome 3
Location:1992988
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g155700 (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAACAACAAGCTGCTGTTGCAGCCCGCTCTGGACCCAGAGAACCTGT
Internal bar code:GGGGCAGATGGCCCGAGTTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2230
LEAP-Seq percent confirming:21.4286
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCATCTGAAGTCCGGCA
Suggested primer 2:GGTGAAACGTCTGAGGCTGA