| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040094 |
| Chromosome: | chromosome 4 |
| Location: | 1710976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212250 | (1 of 82) IPR020846 - Major facilitator superfamily domain; GT77 family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGTCCTCACTGGGCGGCGGCTATGGAGACGAGGTCGGGCTGGGCGG |
| Internal bar code: | TTATAGATATGACGTTGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGATAGCCATTCATGCGA |
| Suggested primer 2: | TGAGTCCCTGAGTCCCTGAG |